Clinical Evidence

Clinical evidence

Dentyl Active has conducted a series of independent studies to demonstrate the benefits of this smart, active mouthwash. Dentyl Active has undergone intensive clinical trialsThe studies completed so far have confirmed the antibacterial activity of Cetylpyridinium chloride (CPC) in an essential oil suspensor, as well as Dentyl Active’s ability to eradicate 99.9% oral bacteria.6  The other area that has been investigated relates to the efficacy of Dentyl Active in reducing malodour. A further clinical study (Rosenberg et al 1992) has also shown that two phase oil: water mouthwash can bring about a significant decrease in volatile sulphides and reduced microbial levels. Trial findings include:

1: Investigation into antimicrobial activity of Dentyl Active mouthwash: research conducted by The Lord Zuckerman Research Centre (dated 10/01/05).

The study undertook a suspension test to show that the sample possesses an inherent antimicrobial effect (in vitro). It also looked at total counts to show that the sample reduces the level of micro-organisms in the mouth (in vivo).

It revealed a significant reduction in the level of viable micro-organisms expelled within saliva into the mouthwash, compared with the level expelled in saliva in the presence of water. This confirms that the mouthwash is having an anti-microbial effect.

2: Two phase mouthwash formulations for treatment of oral malodour; Authors O.Ilan; A.Kozlovsky, S.Goldberg, E.I. Weiss and M.Rosenberg. pp. 265-274.

In bad breath: A multidisciplinary approach; D.van Steenberghe and M.Rosenberg (eds). Leuven University Press, Leuven, Belgium;

This study tested the effectiveness of the Cetylpyridinium chloride (CPC) element of Dentyl mouthwash using control test tubes.

The results demonstrated that oil: water mixtures containing CPC enhance the activity of the antibacterial agent by prolonging its release over time. The vital ingredient is CPC, which was proven to exhibit long-term reduction of microbial levels and malodour scores. This has been attributed to the ability of the oil droplets to bind and remove oral micro-organisms and debris.

3: Effects of a two phase oil: water mouthwash on halitosis – Clinical Preventive Dentistry; Authors – Ken Yaegaki, DDS, PhD and Kazou Sanada, DDS DDSc+; Clinical Preventive Dentistry, Vol.14, No 1; Jan/Feb 1992.

This study was put into place to determine the effects of Dentyl on the production of volatile sulphides both in vivo and in vitro.

It found that a reduction of 80% sulphide was observed using a two phase mouthwash, compared to 30% when other commercial mouthwashes were used. The clinical test concluded that the two phase mouthwash, Dentyl, strongly inhibits the production of volatile sulphides.

4: Assessing the antimicrobial properties of Dentyl Active mouthwash when challenged with bacteria commonly found in the oral cavity; December 2008; independent data on file.

The study determined the effects of Dentyl on organisms commonly associated with gingivitis using a kill kinetics model in vitro, to evaluate the hostility of a mouthwash containing a high bioavailable CPC to five organisms commonly associated with plaque and gingivitis (Candida Albicans, Actynomyces Viscosus, Streptoccoccus Sanguinis, Salmonella Typhimurium and Protella Intermedia). The kill kinetics assay is based on the time taken to kill the representative micro-organism.

The study data revealed that 99.9% of germs were killed after 30 seconds of exposure when various formulas of Dentyl containing 0.065% to 0.07% CPC were used.

The clinical test concluded that six different formulas of the two phase mouthwash Dentyl each kill 99.9% of five key bacteria commonly found in the mouth and associated with plaque and gingivitis after just 30 seconds, indicating that Dentyl could be effective in the treatment and prevention of gingivitis.

5: Bacterial desorption by commercial mouthwashes versus two phase oil: water formulations; Authors – S. Goldberg and M.Rosenberg.

Research Centre – The Maurice and Gabriela Goldschleger School of Dental Medicine and Department of Human Microbiology, Sackler Faculty of Medicine,  Tel-Aviv University, Ramat-Aviv, 69978, Tel-Aviv, Israel; published Biofouling, 1991, Vol 3, p. 193-198

Building on past investigations regarding oral micro-organisms, this study investigated the bacterial desorption qualities of various commercial mouthwashes versus a two phase oil: water formulation – Dentyl.

The study data revealed that certain concentrations of cationic antibacterial agents i.e. CPC and Chlorhexidine (CHX) promote binding of oral and other micro-organisms to oil droplets. This has resulted in the development of novel aqueous: oil mouthwashes containing CPC and/or CHX. In a comparison it was found that oil: water mouthwashes had desorption properties far superior to commercial mouthwashes.

6: Day-long reduction of oral malodour by a two phase oil: water mouthrinse as compared to Chlorhexidine and placebo rinses; Authors: M. Rosenberg, I. Gelernter, M. Barki, and R. Barness; Journal of Periodontal 1992; 63:39-43.

Sixty dental students were divided randomly into three groups and instructed to use one of the rinses prior to bedtime and the following morning. Measurements carried out eight to ten hours following rinsing were compared with baseline measurements from the previous day.

The results found that Chlorhexidine and two phase mouthrinse were highly effective in reducing microbial levels as measured by the rinsing technique. The data showed that day long reduction of malodorous sulphides and microbial levels by a two phase mouthrinse extend previous vitro studies and thus demonstrate its potent microbial desorption properties. This type of study provides the first evidence for its potential clinical efficacy.

7: Managing halitosis with CPC mouthwashes; Author: C. Scully, S Porter, J Greenman; (2006).

The amounts of volatile sulphur compounds and the ratio of Methylmercaptan to Hydrogen Sulphide are higher in the mouth air from a patient with periodontal disease than in that from people with healthy mouths.  The source of these compounds is mainly from the gingival crevice, periodontal pockets and the tongue coating.  As a result, this study investigated the management of halitosis with CPC containing mouthwashes.

It found that most reliable management in reducing oral flora, particularly anaerobes, is best achieved by improving oral hygiene i.e. brushing teeth, cleaning between the teeth and other means. A simple and effective treatment is to use a mouthrinse of 0.2% aqueous Chlorhexidine Gluconate, which is remarkably active against a range of organisms in dental plaque and can also reduce halitosis. Various other products designed to reduce halitosis are under development. For example Cetylpyridinium chloride and a two phase oil-water mouthwash containing olive oil and other essential oils reduce volatile sulphur compounds in the breath.